Recombinant BMP-3 (Bone morphogenetic protein-3), Human, Animal-Free

Osteogenin, BMP-3A

Human

His-tag at the N-terminus

Escherichia coli

Human

Musculoskeletal & Connective Tissue Research

<0.1 EU per 1 μg of the protein by the LAL method.

>95% as determined by SDS-PAGE. Ni-NTA chromatography.

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is <9.5 ng/mL.

Lyophilized

Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.

For research use only.

Cell Culture, ELISA

The protein was lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 3.5.

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