Recombinant BMP-4 (Bone morphogenetic protein-4), Human, Animal-Free

BMP-2B, DVR4

Human

His-tag at the N-terminus

Escherichia coli

Human

Stem Cell & Developmental Biology; Musculoskeletal & Connective Tissue Research

<0.1 EU per 1 μg of the protein by the LAL method.

>95% as determined by SDS-PAGE. Ni-NTA chromatography.

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is <0.58 ng/mL.

Lyophilized

Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.

For research use only.

Cell Culture, ELISA

The protein was lyophilized from a solution containing 20 mM sodium carbonate, pH 9.0.

MKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR with polyhistidine tag at the C-terminus.

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