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Recombinant EGF (Epidermal growth factor), Human, Animal-Free

Recombinant EGF (Epidermal growth factor), Human, Animal-Free

Product Specifications

Product Name Alternative

Urogastrone, URG

Reactivity

Human

Tag

His-tag at the N-terminus

Source

Escherichia coli

Origin

Human

Field of Research

Cancer Biology; Stem Cell & Developmental Biology

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Purity

>95% as determined by SDS-PAGE. Ni-NTA chromatography.

Bioactivity

Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 0.05-0.12 ng/mL. The specific activity of recombinant human EGF is approximately >1.4 x 106 IU/mg.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.

Notes

For research use only.

Tested Applications

Cell Culture, ELISA

Preservative

The protein was lyophilized from a solution containing 1X PBS, pH 8.0.

AA Sequence

MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRLE with polyhistidine tag at the C-terminus.

Entrez

1950

Available Sizes

Frequently Asked Questions

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