Recombinant EGF (Epidermal growth factor), Human, Animal-Free
Recombinant EGF (Epidermal growth factor), Human, Animal-Free
Product Specifications
Product Name Alternative
Urogastrone, URG
Reactivity
Human
Tag
His-tag at the N-terminus
Source
Escherichia coli
Origin
Human
Field of Research
Cancer Biology; Stem Cell & Developmental Biology
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Purity
>95% as determined by SDS-PAGE. Ni-NTA chromatography.
Bioactivity
Measure by its ability to induce 3T3 cells proliferation. The ED50 for this effect is 0.05-0.12 ng/mL. The specific activity of recombinant human EGF is approximately >1.4 x 106 IU/mg.
Form
Lyophilized
Storage Conditions
Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Notes
For research use only.
Tested Applications
Cell Culture, ELISA
Preservative
The protein was lyophilized from a solution containing 1X PBS, pH 8.0.
AA Sequence
MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELRLE with polyhistidine tag at the C-terminus.
Entrez
1950
Available Sizes
Frequently Asked Questions
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