Recombinant HMGB1 (High mobility group box 1), Human, Animal-Free

HMG-1, HMG1, HMG3, SBP-1

Human

His-tag at the N-terminus

Escherichia coli

Human

<0.1 EU per 1 μg of the protein by the LAL method.

>98% as determined by SDS-PAGE. Ni-NTA chromatography.

Measure by its ability to induce TNF alpha in RAW264.7 cells. The ED50 for this effect is <10 μg/mL.

Lyophilized

Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.

For research use only.

Cell Culture, ELISA

The protein was lyophilized from a solution containing 1X PBS, pH 8.0.

3146