Recombinant IL-1 beta (Interleukin-1 beta), Human, Animal-Free
Recombinant IL-1 beta (Interleukin-1 beta), Human, Animal-Free
Product Specifications
Product Name Alternative
Catabolin, Lymphocyte-Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endoge_x005F_x0002_nous Mediator (LEM), Mononuclear Cell Factor (MCF)
Reactivity
Human
Tag
His-tag at the N-terminus
Source
Escherichia coli
Origin
Human
Field of Research
Immunology & Inflammation
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Purity
>98% as determined by SDS-PAGE. Ni-NTA chromatography.
Bioactivity
Measure by its ability to induce proliferation in D10.G4.1 cells. The ED50 for this effect is 1.5 x 108 IU/mg. Measure by its ability to induce IL-8 secretion in HT29 cells. The ED50 for this effect is 1.8-5.1 ng/mL.
Form
Lyophilized
Storage Conditions
Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Notes
For research use only.
Tested Applications
Cell Culture, ELISA
Preservative
The protein was lyophilized from a solution containing 1X PBS, pH 8.0.
AA Sequence
MASAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS with polyhistidine tag at the C-terminus.
Entrez
3553
Available Sizes
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