Recombinant BDNF (Brain-derived neurotrophic factor), Human/Mouse, Animal-Free
Recombinant BDNF (Brain-derived neurotrophic factor), Human/Mouse, Animal-Free
Product Specifications
Product Name Alternative
ANON2, BULN2
Reactivity
Human, Mouse
Tag
His-tag at the N-terminus
Source
Escherichia coli
Origin
Human/Mouse
Field of Research
Neuroscience
Endotoxin
<0.1 EU per 1 μg of the protein by the LAL method.
Purity
>98% as determined by SDS-PAGE. Ni-NTA chromatography.
Bioactivity
Measure by its ability to induce proliferation in BaF3 cells transfected with TrkB. The ED50 for this effect is <2 ng/mL.
Form
Lyophilized
Storage Conditions
Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
Notes
For research use only.
Tested Applications
Cell Culture, ELISA
Preservative
The protein was lyophilized from a solution containing 20 mM sodium citrate, 0.2 MNaCl, pH 3.5.
AA Sequence
MHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR with polyhistidine tag at the C-terminus.
Entrez
627
Available Sizes
Frequently Asked Questions
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