Recombinant BDNF (Brain-derived neurotrophic factor), Human/Mouse, Animal-Free

ANON2, BULN2

Human, Mouse

His-tag at the N-terminus

Escherichia coli

Human/Mouse

Neuroscience

<0.1 EU per 1 μg of the protein by the LAL method.

>98% as determined by SDS-PAGE. Ni-NTA chromatography.

Measure by its ability to induce proliferation in BaF3 cells transfected with TrkB. The ED50 for this effect is <2 ng/mL.

Lyophilized

Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.

For research use only.

Cell Culture, ELISA

The protein was lyophilized from a solution containing 20 mM sodium citrate, 0.2 MNaCl, pH 3.5.

627