Recombinant CCL4 (C-C Motif Chemokine Ligand 4), Mouse, Animal-Free

MIP-1b: Macrophage Inflammatory Protein-1β, ACT-2

Mouse

His-tag at the N-terminus

Escherichia coli

Mouse

Immunology & Inflammation

<0.1 EU per 1 μg of the protein by the LAL method.

>98% as determined by SDS-PAGE. Ni-NTA chromatography.

Measure by its ability to chemoattract BaF3 cells transfected with human CCR5. The ED50 for this effect is <4 ng/mL.

Lyophilized

Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.

For research use only.

Cell Culture, ELISA

The protein was lyophilized from a solution containing 1X PBS, pH 7.4.

APMGSDPPTSCCFSYTSRQLHRSFVMDYYETSSLCSKPAVVFLTKRGRQICANPSEPWVTEYMSDLELN with polyhistidine tag at the N-terminus.

20303