Recombinant HMGB1 (High mobility group box 1), Mouse, Animal-Free

HMG-1, Hmg1, SBP, SBP-1, amph, p3, p30

Mouse

His-tag at the N-terminus

Escherichia coli

Mouse

<0.1 EU per 1 μg of the protein by the LAL method.

>95% as determined by SDS-PAGE. Ni-NTA chromatography.

Measure by its ability to induce TNF alpha in RAW264.7 cells. The ED50 for this effect is <15 ng/mL.

Lyophilized

Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.

For research use only.

Cell Culture, ELISA

The protein was lyophilized from a solution containing 1X PBS, pH 7.4.

15289