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Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, Animal-Free

Recombinant TNF alpha (Tumor necrosis factor alpha), Mouse, Animal-Free

Product Specifications

Product Name Alternative

TNFSF2, Cachectin, Differentiation-inducing factor (DIF), Necrosin, Cytotoxin, TNS_x005F_x0002_F1A

Reactivity

Mouse

Tag

His-tag at the N-terminus

Source

Escherichia coli

Origin

Mouse

Field of Research

Cancer Biology; Immunology & Inflammation

Endotoxin

<0.1 EU per 1 μg of the protein by the LAL method.

Purity

>98% as determined by SDS-PAGE. Ni-NTA chromatography.

Bioactivity

Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED50 for this effect is 2.5x 107 IU/mg.

Form

Lyophilized

Storage Conditions

Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.

Notes

For research use only.

Tested Applications

Cell Culture, ELISA

Preservative

The protein was lyophilized from a solution containing 1X PBS, pH 8.0.

AA Sequence

MLRSSSQNSSDKPVAHVVANHQVEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLVYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVNLLSAVKSPCPKDTPEGAELKPWYEPIYLGGVFQLEKGDQLSAEVNLPKYL DFAESGQVYFGVIAL with polyhistidine tag at the C-terminus.

Entrez

21926

Available Sizes

Frequently Asked Questions

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