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ATG16L1 Antibody

Goat polyclonal antibody to ATG16L1

Product Specifications

Product Name Alternative

Anti ATG16 antibody, anti autophagy related 16-like 1 (S. cerevisiae) antibody, anti hCG_1817841 antibody, anti APG16L antibody, anti ATG16L antibody, anti FLJ00045 antibody, anti FLJ10035 antibody, anti FLJ10828 antibody, anti FLJ22677 antibody, anti WDR30 antibody, anti APG16 autophagy 16-like antibody, anti APG16L beta antibody, anti ATG16 autophagy related 16-like protein 1 antibody, anti WD repeat domain 30 antibody

Reactivity

Human, Mouse

Target

ATG16L1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

KVEKVLSKQHSSSIN

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.01-0.1 μg/ml, IHC-P: 3.75 μg/ml

Molecular Weight

68.3, 66.3

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 48kDa band in Human Kidney, Liver and Spleen lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 68.3kDa according to NP_110430.5 and of 66.3kDa according to NP_060444.3. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Canine, Rat

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

55054

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