CHRNB4 Antibody
Goat polyclonal antibody to CHRNB4
Product Specifications
Product Name Alternative
Anti CHRNB4 antibody, anti cholinergic receptor antibody, anti nicotinicN: beta 4 antibody, anti Cholinergic receptor, neuronal nicotinic, beta polypeptide-4 antibody, anti cholinergic receptor, nicotinic, beta polypeptide 4 antibody, anti neuronal nicotinic receptor beta 4 subunit antibody
Reactivity
Human
Target
CHRNB4
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
DYRLTWNSSRYEGVN
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 0.2 μg/ml, IHC-P: 2.5 μg/ml
Molecular Weight
56.4
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 40kDa band in human testis lysates after 0.2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kDa according to NP_000741.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
1143
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