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CHRNB4 Antibody

Goat polyclonal antibody to CHRNB4

Product Specifications

Product Name Alternative

Anti CHRNB4 antibody, anti cholinergic receptor antibody, anti nicotinicN: beta 4 antibody, anti Cholinergic receptor, neuronal nicotinic, beta polypeptide-4 antibody, anti cholinergic receptor, nicotinic, beta polypeptide 4 antibody, anti neuronal nicotinic receptor beta 4 subunit antibody

Reactivity

Human

Target

CHRNB4

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

DYRLTWNSSRYEGVN

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 0.2 μg/ml, IHC-P: 2.5 μg/ml

Molecular Weight

56.4

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 40kDa band in human testis lysates after 0.2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kDa according to NP_000741.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

1143

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