Goat anti-MYF5 Antibody
Goat polyclonal antibody to MYF5
Product Specifications
Product Name Alternative
Anti MYF5 antibody, anti myogenic factor 5 antibody
Reactivity
Human
Target
MYF5
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
PECNSPVWSRKSST
Field of Research
Stem Cell & Developmental Biology; Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.5-1.5 μg/ml
Molecular Weight
28.4
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 28kDa band observed in Human Skeletal Muscle lysates (calculated MW of 28.4kDa according to NP_005584.1) . Recommended concentration: 0.5-1.5 μg/ml. An additional band of 26kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
4617
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