Anti-MAP-1 Antibody

Rabbit Polyclonal Antibody specific to MAP-1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

MAP-1, MAP1, Paraneoplastic antigen Ma4

Gene Name

MOAP1

Gene ID

64112

NCBI Gene ID

UniProt

Q96BY2

Accession Number

NP_071434.2

Cellular Locus

Cytoplasm, cytosol, Mitochondrion outer membrane, Extracellular vesicle membrane

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to aa 337-351 of human MAP-1.

Target Antigen

Modulator of apoptosis 1

Target

MAP-1

Clonality

Polyclonal

Type

Antibody

Applications

ELISA

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Retrotransposon-derived protein that forms virion-like capsids (By similarity). Acts as an effector of BAX during apoptosis: enriched at outer mitochondria membrane and associates with BAX upon induction of apoptosis, facilitating BAX-dependent mitochondrial outer membrane permeabilization and apoptosis (PubMed:11060313, PubMed:16199525). Required for death receptor-dependent apoptosis (PubMed:11060313). When associated with RASSF1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation (PubMed:15949439). Also promotes autophagy: promotes phagophore closure via association with ATG8 proteins (PubMed:33783314). Acts as an inhibitor of the NFE2L2/NRF2 pathway via interaction with SQSTM1: interaction promotes dissociation of SQSTM1 inclusion bodies that sequester KEAP1, relieving inactivation of the BCR(KEAP1) complex (PubMed:33393215). {UniProtKB:Q9ERH6, PubMed:11060313, PubMed:15949439, PubMed:16199525, PubMed:33393215, PubMed:33783314}.

Additionnal Information

Immunoblotting: use at 1:500-1:1,000 dilution. A band of approx. 39kDa is detected. Positive control: Heart or brain tissue lysate. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human MAP-1 (Modulator of Apoptosis Protein), a novel Bax-associating protein. MAP-1 mediates caspase-dependent apoptosis in mammalian cells when overexpressed. MAP-1 homodimerizes and associates with the proapoptotic Bax and the prosurvival Bcl-2 and Bcl-xL of the Bcl-2 family.