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Goat anti-Arylsulfatase D Antibody

Goat polyclonal to Arylsulfatase D

Product Specifications

Product Name Alternative

Anti arylsulfatase D antibody, anti ARSD antibody

Reactivity

Human

Target

Arylsulfatase D

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

DASNGYRALQWNA

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:16000, WB: 0.3-1 μg/ml

Molecular Weight

64.9; 42,3

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

This antibody is expected to recognise both reported isoforms (NP_001660.2; NP_033667.2) ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 60+40kDa bands observed in lysates of cell lines A549 and HepG2 (calculated MW of 64.9kDa according to NP_001660.2 and of 42.3 according to NP_033667.2) . Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

414

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