Goat anti-Arylsulfatase D Antibody
Goat polyclonal to Arylsulfatase D
Product Specifications
Product Name Alternative
Anti arylsulfatase D antibody, anti ARSD antibody
Reactivity
Human
Target
Arylsulfatase D
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
DASNGYRALQWNA
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 0.3-1 μg/ml
Molecular Weight
64.9; 42,3
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This antibody is expected to recognise both reported isoforms (NP_001660.2; NP_033667.2) ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 60+40kDa bands observed in lysates of cell lines A549 and HepG2 (calculated MW of 64.9kDa according to NP_001660.2 and of 42.3 according to NP_033667.2) . Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
414
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