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Recombinant Human Novel Coronavirus Nucleoprotein (N) (P13L) (Active)

This Recombinant Human Novel Coronavirus Nucleoprotein (N) (P13L) (Active) spans the amino acid sequence from region 1-419aa (P13L) . Purity: Greater than 93% as determined by SDS-PAGE.

Product Specifications

UniProt

P0DTC9

Expression Region

1-419aa (P13L)

Tag

N-terminal 6xHis-tagged

Source

E.coli

Origin

Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)

Field of Research

Infectious Disease & Virology

Purity

Greater than 93% as determined by SDS-PAGE.

Bioactivity

1Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Mouse Monoclonal Antibody, the EC50 of N Mouse Monoclonal Antibody is 3.246-4.503 ng/ml. 2Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody is 2.825-4.124 ng/ml.

Form

Lyophilized powder

Molecular Weight

49.7 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Notes

For research use only.

Applications Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Preservative

Lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

AA Sequence

MSDNGPQNQRNALRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA

Available Sizes

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