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NOVA1 Antibody

Goat polyclonal antibody to NOVA1

Product Specifications

Product Name Alternative

Anti NOVA1 antibody, anti neuro-oncological ventral antigen 1 antibody, anti Nova-1 antibody, anti paraneoplastic Ri antigen antibody, anti ventral neuron-specific protein 1 antibody

Reactivity

Human

Target

NOVA1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

REMPQNVAKTEPVS

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx. 50-55kDa band observed in Human Breast Cancer lysates, and in preliminary testing of Human Kidney lysate (calculated MW of 51.7kDa according to NP_002506.2) . Recommended concentration: 0.01-0.03μg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Brain (Cortex) and Heart. Recommended concentration: 5μg/ml. Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.

Molecular Weight

51.7, 19.5

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Tested Applications

ELISA, FC, IF, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

4857

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