NOVA1 Antibody
Goat polyclonal antibody to NOVA1
Product Specifications
Product Name Alternative
Anti NOVA1 antibody, anti neuro-oncological ventral antigen 1 antibody, anti Nova-1 antibody, anti paraneoplastic Ri antigen antibody, anti ventral neuron-specific protein 1 antibody
Reactivity
Human
Target
NOVA1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
REMPQNVAKTEPVS
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx. 50-55kDa band observed in Human Breast Cancer lysates, and in preliminary testing of Human Kidney lysate (calculated MW of 51.7kDa according to NP_002506.2) . Recommended concentration: 0.01-0.03μg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Brain (Cortex) and Heart. Recommended concentration: 5μg/ml. Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.
Molecular Weight
51.7, 19.5
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Tested Applications
ELISA, FC, IF, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
4857
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