Recombinant beta-NGF (Nerve growth factor-beta), Mouse, Animal-Free

β-Nerve Growth Factor, NGF-β

Mouse

His-tag at the N-terminus

Escherichia coli

Mouse

Neuroscience; Stem Cell & Developmental Biology

<0.1 EU per 1 μg of the protein by the LAL method.

>98% as determined by SDS-PAGE. Ni-NTA chromatography.

Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is 1 x 106 IU/mg.

Lyophilized

Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.

For research use only.

Cell Culture, ELISA

The protein was lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5.

18049