Recombinant BMP-4 (Bone morphogenetic protein-4), Mouse, Animal-Free

BMP-2B, DVR4

Mouse

His-tag at the N-terminus

Escherichia coli

Mouse

Stem Cell & Developmental Biology; Musculoskeletal & Connective Tissue Research

<0.1 EU per 1 μg of the protein by the LAL method.

>98% as determined by SDS-PAGE. Ni-NTA chromatography.

Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED50 for this effect is 1 x 105 IU/mg.

Lyophilized

Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.

For research use only.

Cell Culture, ELISA

The protein was lyophilized from a solution containing 20 mM sodium carbonate, pH 4.5.

MKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCRwith polyhistidine tag at the C-terminus.

12159