COL4A3BP (aa662-672) Antibody

Goat polyclonal antibody to Collagen IV

Product Specifications

Product Name Alternative

Anti-Arresten antibody, anti-COL4A1 antibody, anti-COL4A1 NC1 domain antibody, anti-COL4A2 antibody, anti-COL4A3 antibody, anti-COL4A4 antibody, anti-COL4A5 antibody, anti-collagen alpha-1 (IV) chain antibody, anti-Collagen IV Alpha 1 Polypeptide antibody, anti-Collagen IV Alpha 2 Polypeptide antibody, anti-Collagen Of Basement Membrane Alpha 1 Chain antibody, anti-Collagen Of Basement Membrane Alpha 2 Chain antibody, anti-Collagen Type IV Alpha 1 antibody, anti-Collagen Type IV Alpha 2 antibody, anti-Collagen Type IV Alpha 3 antibody, anti-Collagen Type IV Alpha 4 antibody, anti-Collagen Type IV Alpha 5 antibody, anti-Collagen Type IV antibody

Reactivity

Mouse

Target

COL4A3BP (aa662-672)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

DHDSAPLNNRC

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.3-1 μg/ml

Molecular Weight

70.8; 60.0

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60kDa band observed in mouse heart lysates (calculated MW of 71.1kDa according to mouse NP_075909.1 and 60.0kDa according to human NP_112729.1) . Recommended concentration: 0.5-1.5 μg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Human

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.