P2RY1 (aa247-257) Antibody
Goat polyclonal antibody to P2RY1
Product Specifications
Product Name Alternative
Anti P2RY1 antibody, anti purinergic receptor P2Y, G-protein coupled, 1 antibody, anti P2Y1 antibody, anti ATP recepto antibody, anti P2 purinoceptor subtype Y1 antibody, anti P2Y purinoceptor 1 antibody, anti platelet ADP receptor antibody, anti purinergic receptor P2Y1 antibody
Target
P2RY1 (aa247-257)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
KDLDNSPLRRK
Field of Research
Cardiovascular Research
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.1 μg/ml
Molecular Weight
42.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave bands at approx 55kDa and 38kDa in lysates of cell lines HEK293 and A549 after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 42.1kDa according to NP_002554.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the observed sizes) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Canine, Human, Mouse, Rat
Tested Applications
ELISA
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
5028
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