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P2RY1 (aa247-257) Antibody

Goat polyclonal antibody to P2RY1

Product Specifications

Product Name Alternative

Anti P2RY1 antibody, anti purinergic receptor P2Y, G-protein coupled, 1 antibody, anti P2Y1 antibody, anti ATP recepto antibody, anti P2 purinoceptor subtype Y1 antibody, anti P2Y purinoceptor 1 antibody, anti platelet ADP receptor antibody, anti purinergic receptor P2Y1 antibody

Target

P2RY1 (aa247-257)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

KDLDNSPLRRK

Field of Research

Cardiovascular Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.1 μg/ml

Molecular Weight

42.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave bands at approx 55kDa and 38kDa in lysates of cell lines HEK293 and A549 after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 42.1kDa according to NP_002554.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the observed sizes) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Canine, Human, Mouse, Rat

Tested Applications

ELISA

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

5028

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