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GOT1 (aa 157-167) Antibody

Goat polyclonal to GOT1

Product Specifications

Product Name Alternative

Anti GOT1 antibody, anti glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) antibody, anti GIG18 antibody, anti aspartate aminotransferase 1 antibody, anti growth-inhibiting protein 18 antibody

Reactivity

Human

Target

GOT1 (aa 157-167)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RSYRYWDAEKR

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:4000, WB: 0.01-1 μg/ml, IHC-P: 5 μg/ml

Molecular Weight

46.2

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: In paraffin embedded Human Heart shows strong staining of the capillary network between the myocardial fibres in transverse secion. Recommended concentration, 2-3ug/ml.WB: Approx 38-40kDa band observed in Human Brain (Cerebellum), Liver and Skeletal Muscle lysates (calculated MW of 46.2kDa according to NP_002070.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Berk et al, Proc Natl Acad Sci U S A. 1990 May;87 (9) :3484-8; PMID: 2185471) . Recommended concentration: 0.01-1 μg/ml.

Prediction Reactivity

Rat

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

2805

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