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Ribosomal protein L22 Antibody

Goat polyclonal antibody to RPL22

Product Specifications

Product Name Alternative

Anti RPL22 antibody, anti ribosomal protein L22 antibody, anti EAP antibody, anti HBP15 antibody, anti HBP15/L22 antibody, anti 60S ribosomal protein L22 antibody, anti EBER-associated protein antibody, anti Epstein-Barr virus small RNA-associated protein antibody, anti Epstein-Barr-encoded RNA-associated protein antibody, anti heparin-binding protein 15 antibody, anti heparin-binding protein HBp15 antibody

Reactivity

Human

Target

Ribosomal protein L22

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

SKESYELRYFQINQ

Field of Research

Epigenetics

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

3-6 μg/ml

Dilution

ELISA: 1:16000, WB: 0.5 μg/ml, IHC-P: 3.75 μg/ml

Molecular Weight

14.8

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave bands at approx 17-18kDa and 25kDa in Human Liver lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.8kDa according to NP_000974.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Mouse, Rat

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

6146

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