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CRP2/CSRP2 Antibody

Goat polyclonal antibody to CSRP2

Product Specifications

Product Name Alternative

Anti CRP2 antibody, anti CSRP2 antibody, anti cysteine and glycine-rich protein 2 antibody, anti LMO5 antibody, anti SmLIM antibody, anti LIM domain only 5, smooth muscle antibody

Reactivity

Human, Mouse, Rat

Target

CRP2 / CSRP2

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

ERLGIKPESVQP

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 3-6 μg/ml

Molecular Weight

21

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 26kDa band observed in Human Heart and Kidney lysates and in Mouse and Rat Kidney lysates (calculated MW of 21.0kDa according to NP_001312.1) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Huber et al, J Biol Chem. 2000 Feb 25;275 (8) :5504-11; PMID: 10681529) . In transfected HEK293 transiently expressing CRP2 a band of approx. 26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3 μg/ml.

Prediction Reactivity

Equine

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

1466

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