Anti-Hsp22 Antibody

Mouse Monoclonal Antibody specific to Hsp22

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Hsp22 Mouse Monoclonal Antibody

Gene Name

HSPB8, SLC29A1

Gene ID

26353, 26354

NCBI Gene ID

UniProt

Q9UJY1,Q9UJY2

Accession Number

NP_055180.1, NP_055180.2

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

His-tagged recombinant human Hsp22

Target Antigen

Heat shock protein beta-8, Equilibrative nucleoside transporter 1

Target

Hsp22

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

Field of Research

Heat Shock& Stress Proteins

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at 1-10ug/ml. A band of ~22kDa (monomeric) is detected. <br><br>These are recommended concentrations. User should determine optimal concentrations for their application. <br><br>Positive control: Whole rat tissue extract.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat Hsp22. It detects endogenous and exogenous Hsp22 in monomeric, dimeric, and tetrameric (weak) forms in Western blots. Exogenous Hsp22 is detected in dimeric form.It does not cross-react with alpha crystallin.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Hsp22 (HspB8) is a member of the small heat shock protein super-family. Like most other sHsps, Hsp22 is predominantly transcribed in skeletal muscle and heart as well as placenta. It is a monomeric protein which interacts with HspB1. It displays temperature- dependent chaperone activity. Hsp22 has two binding domains (N- and C- terminal) that are specific for different binding partners and has the ability to bind itself and other sHsps. Its chaperone-like activity is important to the function of Hsp22 in various processes including proliferation, apoptosis, and macroautophagy.