Recombinant Stenotrophomonas maltophilia Elongation factor G (fusA), partial
Product Specifications
Product Name Alternative
EF-G
Abbreviation
Recombinant Stenotrophomonas maltophilia fusA protein, partial
Gene Name
FusA
UniProt
B4SKW0
Expression Region
8-290aa
Organism
Stenotrophomonas maltophilia (strain R551-3)
Target Sequence
ERYRNFGIMAHIDAGKTTTSERILFYTGKSHKIGEVHDGAATMDWMEQEQERGITIQSAATTAFWKGMDKSLPEHRFNIIDTPGHVDFTIEVERSLRVLDGAVFVLCAVGGVQPQSETVWRQANRYKVPRIAFVNKMDRTGANFYKVRDQLKAKLGAVAVPMQLPIGAEEGFKGVVDLLKMKAIHWDEASQGMKFEYGDIPADLQEKAEEARTFMIEAAAEANEELMEKYLGGEELAEAEIINALRTRTLATEIVPMYCGSAFKNKGVQAMLDGVIQLLPSPV
Tag
C-terminal 6xHis-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Others
Relevance
Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
38.4 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial
Available Sizes
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