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Goat anti-CRHR1 / CRF-R (aa 107 - 117) Antibody

Goat polyclonal antibody to CRHR1

Product Specifications

Product Name Alternative

Anti CRHR1 antibody, anti CRF-R antibody, anti corticotropin releasing hormone receptor 1 antibody, anti CRF1 antibody, anti CRFR1 antibody, anti CRH-R1h antibody, anti CRHR antibody, anti CRHR1f antibody, anti corticotropin releasing hormone receptor variant 1h antibody, anti seven transmembrane helix receptor antibody

Reactivity

Human, Mouse, Rat

Target

CRHR1 / CRF-R (aa 107 - 117)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

NEEKKSKVHYH

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 10 μg/ml

Molecular Weight

47.7

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: This product was successfully used on cryosection of mouse brain (PMID: 22570383) WB: Approx 60-70kDa band observed in human brain (cerebellum) and human colon lysates (calculated MW of 47.7kDa according to NP_004373.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Xu et al, Mol Pharmacol. 2001 Mar;59 (3) :485-92; PMID: 11179443) . Recommended concentration: 0.1-0.3 μg/ml.Experiment Notes: Immunofluorescence: This product was successfully used in immunofluorescence on mouse brain at 2ug/ml when incubated at 4C for 40h.

Tested Applications

ELISA, IF, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

1394

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