Goat anti-Adenosine A1 receptor Antibody
Goat polyclonal antibody to ADORA1
Product Specifications
Product Name Alternative
Anti ADORA1 antibody, anti adenosine A1 receptor antibody, anti RDC7 antibody
Reactivity
Human
Target
Adenosine A1 receptor
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
NDHFRCQPAPPIDED
Field of Research
Pharmacology & Drug Discovery; Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml
Molecular Weight
36.5; 29.4; 24.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave bands at approx 30kDa and 20kDa in Human Brain (Cerebellum) lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 36.5kDa according to NP_000665.1 and NP_001041695.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Tested Applications
ELISA, FC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
134
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