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Goat anti-Adenosine A1 receptor Antibody

Goat polyclonal antibody to ADORA1

Product Specifications

Product Name Alternative

Anti ADORA1 antibody, anti adenosine A1 receptor antibody, anti RDC7 antibody

Reactivity

Human

Target

Adenosine A1 receptor

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

NDHFRCQPAPPIDED

Field of Research

Pharmacology & Drug Discovery; Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml

Molecular Weight

36.5; 29.4; 24.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave bands at approx 30kDa and 20kDa in Human Brain (Cerebellum) lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 36.5kDa according to NP_000665.1 and NP_001041695.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Tested Applications

ELISA, FC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

134

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