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ABHD4 (aa 177 to 187) Antibody

Goat polyclonal antibody to ABHD4

Product Specifications

Product Name Alternative

Anti ABHD4 antibody, anti abhydrolase domain containing 4 antibody, anti FLJ12816 antibody

Reactivity

Human

Target

ABHD4 (aa 177 to 187)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RPTNPSEIRAP

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:4000, WB: 1 μg/ml, IHC-P: 3.75 μg/ml

Molecular Weight

38.8

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 48kDa band in Human Brain (Cerebellum, Cerebral Cortex and Frontal Cortex) lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.8kDa according to NP_071343.2. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Canine, Mouse

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

63874

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