What is the catalog number for RNase A (DNase free)?

The catalog number for RNase A (DNase free) is orb532708.

What sizes are available for RNase A (DNase free)?

RNase A (DNase free) is available in the following size(s): 2x 250 mg.

Is RNase A (DNase free) in stock?

RNase A (DNase free) is currently made to order / not held in immediate stock. Contact us for lead time.

RNase A (DNase free)

RNase A is an endoribonuclease that attacks at the 3'-phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with ssRNA. RNase A is free of detectable DNase and protease activity, a heat treatment of the enzyme is not necessary before use. Stability: RNase A is an extremely stable enzyme, remarkable resistant to heating. It renatures easily after treatment with most denaturing agents.

Product Specifications

Product Name Alternative

Endoribonuclease, Bovine pancreas

Concentration

Working concentration: 1-100 μg/ml (depending on application) . The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase cleaves ss and dsRNA as well as the RNA strand in RNA-DNA hybrids.

Purity

≥ 90% (ion exchange chromatography), salt free, chromatographically homogeneous lyophilisate

Bioactivity

≥ 80 Kunitz units/mg

Form

Dry powder

Molecular Weight

Theoretical MW: 13.7 kDa (monomer)

Storage Conditions

Store at 2-8 °C

Notes

For research use only.

Applications Notes

Applications: 1) Plasmid and genomic DNA preparation 2) Removal of RNA from recombinant protein preparations 3) Ribonuclease protection assays 4) Mapping single-base mutations in DNA or RNA.