Adenovirus Type 2 Hexon Antigen

Culture system: Hep-2 cell culture. Preparation: Harvested infected cells are lysed, treated with solvent to remove cellular components and ultracentrifuged to remove whole virus particles. Adenovirus Type 2 Hexon antigen is purified from the centrifugation supernatant by ion exchange chromatography. The antigen is presented in 30 mM Bis-Tris buffer at pH 6.8 containing sodium chloride. Inactivation: Whole infective virus particles are removed by ul-tracentrifugation and chromatography. Confirmed inactive by attempted growth under original culture conditions. Since no procedure can guarantee absolute sterility, the reagent should be handled with appropriate safety precautions.