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Estradiol Glow

Estradiol Glow is the ovarian steroid hormone 17-ß-Estradiol labeled with a novel low molecular weight orange/red fluorophor thus retain-ing its chemical properties and it ́s biological activity. This allows for a wide range of applications including: analysis of in vivo andin vitro steroid uptake in real time; monitoring of intracellular and subcellular steroid transport; studies on steroid-binding proteins; use as tracer for steroid immunoassays (to replace isotopes or enzymes; photodynamic elimination of estrogen sensitive tissues and cells in experimental settings with possible future clinical im-plications Estradiol Glow can be histologically fixed using formaldehyde or glutaraldehyde without any significant loss of fluorescence intensity. Typical concentration range used for cell culture experiments: 10-5M-10-7M (may vary depending on cell line and individual experimental set-up) .

Product Specifications

Product Name Alternative

Fluorescently labeled Estradiol

Field of Research

Cancer Biology; Pharmacology & Drug Discovery

Purity

> 97% (DC, NMR)

Form

Lyophilised, glassy to waxy appearance; Color: brownish-red

Solubility

High: MeOH, EtOH, PrOH, DMSO, DMF, Moderate: acetone, CH 2Cl2, CH3Cl, THF, Low: H2O and aqueous buffer systems (after presolution in DMSO > 1 μM), Insoluble: Et 2O, hydrocarbons such as hexane, heptane, benzene

Molecular Weight

Theoretical MW: 771.95 g/mol

Storage Conditions

Store at 4 °C

Notes

For research use only.

Applications Notes

Spectroscopic Propertie: λexc 467 nm, λem 618 nm, ε 29.0 L mmol-1 cm-1 (PBS pH 7.4), λexc 501 nm, λem 596 nm, ε 52.0 L mmol-1 cm-1 (ethanol) .

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