RafRBDGST (C-Raf)

Raf is a member of the serine/threonine proteine kinase family involved in regulation of cell growth and differentiation and is the most important effector of Ras. Full-length Raf is composed of three conserved regions responsible for interaction with Ras, for phos-phorylation and for catalytic activity. RafRBD (Ras binding domain, amino acids 50-132) mediates interaction with membrane-anchored Ras necessary for activation of the kinase activity of Raf. The GST-Tag facilitates the protein‘s application in typical GST pull-down assays. The protein is GST-tagged on its N-terminus. There is a 7 AA linker between GST and RBD.