GGTase-IGST
GGTase-I (Geranylgeranyltransferase-I) catalyzes the transfer of the farnesyl and geranylgeranyl groups from farnesyl and geranylgeranyl-diphosphate to proteins containing a C-terminal CaaX motif, where 'C' is a conserved cysteine that is the site of farnesyl modification, 'a' is usually an aliphatic amino acid, and 'X' is leucine or phenylalanine. Farnesyltransferase (FT) and GGTase-I are closely related, sharing a common α subunit and 30% identity in their β subunits. The GST-Tag facilitates the protein‘s application in typical GST pull-down assays.
Product Specifications
Product Name Alternative
Protein geranylgeranyltransferase type I, rat, recombinant, E. coli
Source
E. coli
Origin
Rat
Purity
> 90% (SDS-PAGE)
Form
Liquid (Supplied in 25 mM HEPES pH 7.2, 25 mM NaCl and 5 mM DTT)
Molecular Weight
Theoretical MW: α: 44 kDa, β: 38 kDa
Storage Conditions
Store at -80 °C
Notes
For research use only.
Applications Notes
Activity: 1 pmol of GGTase-I will transfer 5 pmol of Farnesyl to RhoA in 15 min at 37°C.
Frequently Asked Questions
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