T4 dNMP (deoxy-Nucleotide Monophosphate) Kinase
NMP kinases catalyse the phosphorylation of nucleotide monophos-phates resulting in the corresponding nucleotide diphosphates by using ATP as a phosphate donor. T4 dNMP kinase is the only member of the family of NMP kinases that recognizes three structurally dissimilar nucleotides: dGMP, dTMP and 5-hydroxymethyl-dCMP (5-OH-dCMP) .
Product Specifications
Product Name Alternative
Bacteriophage T4, recombinant, E. coli
Source
E. coli
Origin
Bacteriophage T4
Field of Research
Signal Transduction
Purity
> 90% (SDS-PAGE)
Bioactivity
Specific activity: 100 u/μg.
Form
Liquid (Supplied in 100 mM Tris-HCl pH 7.5, 10 mM MgCl 2and 50 % glycerol)
Molecular Weight
Theoretical MW: 27 kDa
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Activity Assay: 1 μg T4 dNMP kinase, 2 mM ATP and 2 mM dGMP in 100 mM Tris-HCl pH 7.5, 10 mM MgCl 2. Activity: One unit of enzyme catalyses the phosphorylation of 1 nmol dGMP to dGDP per minute at 37°C. Specific activity: 100 u/μg.
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