Human PLAU Protein, His Tag
Human PLAU, His Tag (activated by trypsin) is expressed from human 293 cells (HEK293) . It contains AA Ser 21 - Leu 431 (Accession # P00749-1) .
Product Specifications
UniProt
P00749
Expression Region
Ser 21 - Leu 431
Reactivity
Human
Conjugation
Unconjugated
Tag
C-6xHis
Field of Research
Cardiovascular Research
Endotoxin
1.0 EU per μg
Purity
90%
Form
Powder
Molecular Weight
15.3 kDa, 17.8 kDa and 29.2 kDa
Storage Conditions
-20°C
Notes
For research use only.
Applications Notes
This protein carries a polyhistidine tag at the C-terminus. The active form of Human PLAU is a disulfide-linked heterodimer composed of long chain A (Ser 21 - Phe 177) and chain B (Ile 179 - Leu 431) with calculated MW of 17.8 kDa and 29.2 kDa. The long chain A is further cleaved to yield a short chain A (Lys 156 - Phe 177) and N-terminal fragment (Ser 21 - Lys 155) with calculated MW of 15.3 kDa. The protein migrates as 31-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
NCBI Accession Number
P00749
Preservative
25 mM HEPES, 150 mM NaCl, pH 7.5
Available Sizes
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