Tcfec (mouse) Antibody

Goat polyclonal antibody to Tcfec

Product Specifications

Product Name Alternative

Anti Tcfec antibody, anti transcription factor EC antibody, anti BB107417 antibody, anti MGC107474 antibody, anti TFEC antibody

Reactivity

Human, Mouse

Target

Tcfec (mouse)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

HITKQQTHPERN

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.5 μg/ml, IHC-P: 5 μg/ml

Molecular Weight

35.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave one band at approx 37kDa and much stronger bands at approx 70+28kDa in Mouse Testis lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the stonger bands we observe given the calculated size of 35.1kDa according to Mouse NP_112475.1. All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Rat

Tested Applications

ELISA, IHC

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.