SARS S Protein, His Tag
SARS S protein (R667A, K968P, V969P), His Tag is expressed from human 293 cells (HEK293) . It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P) ) . The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.
Product Specifications
UniProt
P13567
Expression Region
Ser 14 - Pro 1195
Reactivity
Virus
Conjugation
Unconjugated
Tag
C-10xHis
Field of Research
Infectious Disease & Virology
Endotoxin
1.0 EU per μg
Purity
95%
Form
Powder
Molecular Weight
136.3 kDa
Storage Conditions
-20°C
Notes
For research use only.
Applications Notes
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 136.3 kDa. The protein migrates as 190-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
NCBI Accession Number
R667, V969, AAP13567.1, K968
Preservative
50 mM Tris, 150 mM NaCl, 0.2 M Sodium citrate, pH 5.5
Available Sizes
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