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SARS S Protein, His Tag

SARS S protein (R667A, K968P, V969P), His Tag is expressed from human 293 cells (HEK293) . It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P) ) . The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.

Product Specifications

UniProt

P13567

Expression Region

Ser 14 - Pro 1195

Reactivity

Virus

Conjugation

Unconjugated

Tag

C-10xHis

Field of Research

Infectious Disease & Virology

Endotoxin

1.0 EU per μg

Purity

95%

Form

Powder

Molecular Weight

136.3 kDa

Storage Conditions

-20°C

Notes

For research use only.

Applications Notes

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 136.3 kDa. The protein migrates as 190-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

NCBI Accession Number

R667, V969, AAP13567.1, K968

Preservative

50 mM Tris, 150 mM NaCl, 0.2 M Sodium citrate, pH 5.5

Available Sizes

Frequently Asked Questions

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