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Recombinant Streptavidin Protein

Streptavidin is a tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity for biotin. Able to bind one molecule of biotin with each subunit. Streptavidin (PI=6.0-7.5) has lower level of non-specific binding to various biological components at physiological pH than avidin (PI=7.4), resulting from its isoelectric point (PI) . Streptavidin is useful in affinity chromatography, ELISA, immunohistochemistry and Western Blotting.

Product Specifications

Reactivity

Bacteria

Conjugation

Unconjugated

Tag

Native

Endotoxin

1.0 EU per μg

Purity

95%

Form

Powder

Molecular Weight

13.8 kDa

Storage Conditions

-20°C

Notes

For research use only.

Applications Notes

This protein carries no "tag". The protein has a calculated MW of 13.8 kDa. The protein migrates as 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

Preservative

NaCl, pH 7.4

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