B7-H4 Antibody
Goat polyclonal antibody to VTCN1
Product Specifications
Product Name Alternative
Anti B7-H4 antibody, anti B7H4 antibody, anti B7S1 antibody, anti B7X antibody, anti B7h.5 antibody, anti FLJ22418 antibody, anti PRO1291 antibody, anti RP11-229A19.4 antibody, anti VCTN1 antibody, anti T cell costimulatory molecule B7x antibody, anti immune costimulatory protein antibody, anti V-set domain containing T cell activation inhibitor 1 antibody
Reactivity
Human
Target
B7-H4
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
SKGKGNANLEYK
Field of Research
Immunology & Inflammation
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.1-1 μg/ml, IHC-P: 10ug/ml
Molecular Weight
30.9; 20.7
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 60kDa band observed in Human Lung, Spleen and Thymus lysates (calculated MW of 30.9kDa according to NP_078902.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Simon et al, Cancer Res. 2006 Feb 1;66 (3) :1570-5.; PMID: 16452214) . In transfected HEK293 transiently expressing B7-H4 the band of approx. 60kDa is also observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/ml.
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, FC, IF, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
79679
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