CheKine Micro Total Bile Acid (TBA) Content Assay Kit

Total bile acid (TBA) is mainly used for screening and prognostic follow-up of hepatobiliary diseases and used as a marker of liver parenchymal damage and cholestasis. Viral hepatitis, cirrhosis, alcoholic liver disease, drug-induced liver injury, cholestasis, etc. often lead to an increase in serum total bile acid levels. The CheKine Total Bile Acid (TBA) Content Assay Kit can detect the total bile acid content in serum or plasma. The principle is that the bile acid is specifically oxidized by 3 α -hydroxysteroid dehydrogenase (3α -HSD) and oxidized β -thionicotinamide adenine dinucleotide (Thio-NAD+) to generate 3-ketosteroids and reduced β -thionicotinamide adenine dinucleotide (Thio-NADH) . The 3-ketosteroids react with 3 α -HSD and reduced β -nicotinamide adenine dinucleotide (NADH), and generate bile acids and oxidized β-nicotinamide adenine dinucleotide (NAD+) . Within a certain period of time, the Thio-NADH produced by the enzyme cycle is directly proportional to the bile acid content in the sample. By detecting the changes in Thio-NADH absorbance at a wavelength of 405nm, the total bile acid content in the sample can be calculated.