Annexin V-APC/7-AAD Apoptosis Detection Kit

Apoptosis is a form of programmed cell death to remove unwanted, damaged, or senescent cells from tissues. In normal cells, the negative phospholipids reside on the inner side of the cellular membrane while the outer surface of the membrane is occupied by uncharged phospholipids (PS) . After a cell has entered apoptosis, the negatively charged PS are transported from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment. The human anticoagulant, Annexin V, is a 35-36 kDa Ca2+ dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet. 7-AAD is a fluorescent nucleus dye, impermeant to live cells and apoptotic cells, but stains dead cells with red fluorescence, binding tightly to the nucleic acids in the cell. Annexin V-APC/7-AAD Apoptosis Detection kit provides a rapid and convenient assay for apoptosis. After staining a cell population with Annexin V-APC/7-AAD in the provided binding buffer, early apoptotic cells were only positive for Annexin V, dead cells were positive for Annexin V and 7-AAD, and live cells were negative for Annexin V and 7-AAD. In addition, this kit also provides Apoptosis Positive Control Solution specifically for apoptosis detection compensation regulation. Detection can be analyzed using flow cytometry or fluorescence microscopy