Human PD-1 ELISA Kit

Human PD-1 ELISA Kit employs a two-site sandwich ELISA to quantitate PD-1 in samples. An antibody specific for PD-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PD-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PD-1 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EGF bound in the initial step. The color development is stopped and the intensity of the color is measured.