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Rat TNF-α ELISA Kit

Rat TNF-α ELISA Kit employs a two-site sandwich ELISA to quantitate Rat TNF-α.

Product Specifications

Product Name Alternative

TNF-α; TNF; DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha; TNFSF7

Reactivity

Rat

Field of Research

Immunology & Inflammation

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Sensitivity

16 pg/ml

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Notes

For research use only.

Applications Notes

Rat TNF-α ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-α in samples. An antibody specific for TNF-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-α present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-α is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-α bound in the initial step. The color development is stopped and the intensity of the color is measured.

Tested Applications

ELISA

Dynamic Range

31.25 pg/mL- 2000 pg/mL

Available Sizes

Frequently Asked Questions

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