Rat TNF-α ELISA Kit
Rat TNF-α ELISA Kit employs a two-site sandwich ELISA to quantitate Rat TNF-α.
Product Specifications
Product Name Alternative
TNF-α; TNF; DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha; TNFSF7
Reactivity
Rat
Field of Research
Immunology & Inflammation
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Sensitivity
16 pg/ml
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Notes
For research use only.
Applications Notes
Rat TNF-α ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-α in samples. An antibody specific for TNF-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-α present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-α is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-α bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA
Dynamic Range
31.25 pg/mL- 2000 pg/mL
Available Sizes
Frequently Asked Questions
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