Mouse IL-10 ELISA Kit

Mouse IL-10 ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-10.

Product Specifications

Product Name Alternative

IL 10; IL10; CSIF; IL-10; IL10A; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor

Reactivity

Mouse

Field of Research

Immunology & Inflammation

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Sensitivity

50 pg/mL

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Notes

For research use only.

Applications Notes

Mouse IL-10 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-10 in samples. An antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-10 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound in the initial step. The color development is stopped and the intensity of the color is measured.