Mouse IFN-γ ELISA Kit

Mouse IFN-γ ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IFN-γ.

Product Specifications

Product Name Alternative

IFG; IFI; IFN gamma; IFN Immune; IFNG; Interferon gamma; Interferon Gamma Precursor; Macrophage Activating Factor; MAF; T Cell Interferon; Type II Interferon

Reactivity

Mouse

Field of Research

Infectious Disease & Virology

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Sensitivity

16 pg/ml

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Notes

For research use only.

Applications Notes

Mouse IFN-γ ELISA Kit employs a two-site sandwich ELISA to quantitate IFN-γ in samples. An antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-γ is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-γ bound in the initial step. The color development is stopped and the intensity of the color is measured.